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Molecular Genetics

MLPA

Multiplex Ligation-Dependent Probe Amplication is a Multiplex PCR method that enables the detection of the Abnormal Copies Number in the genomic DNA or RNA sequence to allow separation even in sequences with a single nucleotide change. MLPA is more sensitive than other techniques in determining the number of copies. Although well-characterized deletions and amplifications can be detected by PCR, the exact breakpoints of the deletions are often unknown. Frequent single gene changes, which cannot be detected by the FISH method, are very advantageous in determining short sequences such as 50-70 nucleotides and are therefore highly preferred and used.

MPLA Usage Areas:

  • Mutation analysis
  • Single nucleotide polymorphism
  • DNA methylation analysis
  • mRNA concentration
  • Chromosome analysis of cell lines and tissues
  • Analysis of gene copy number
  • Detection of duplication and deletions in cancer genes
  • Aneuploidy detection
  • Prenetal diagnosis etc.